0704-883-0675     |      dataprojectng@gmail.com

ANTIMICROBIAL SUSCEPTIBILITY AND MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS FROM MASTITIC COWS

  • Project Research
  • 1-5 Chapters
  • Abstract : Available
  • Table of Content: Available
  • Reference Style: APA
  • Recommended for : Student Researchers
  • NGN 5000

ABSTRACT

This study investigated the antimicrobial susceptibility and molecular characterization of Staphylococcus aureus in mastitic cows in southern part of Kano State of Nigeria. A total of two hundred and thirty four (234) milk and swab samples were collected from six (6) local government areas of Kano State in northern part of Nigeria. Staphylococcus aureus was isolated from samples and identified using standard microbiological procedures. The antimicrobial susceptibility of the isolates was determined using Kirby- Bauer disc diffusion technique as described by the Clinical and Laboratory standards institute (CLSI). Resistant isolates were tested for Beta-lactamase production. A total of 200 (85.5%) have staphylococcal isolates, of which 141 (70.5%) were Staphylococcus aureus (coagulase positive staphylococci, CPS), 59 (29.5%) were coagulase negative staphylococci (CoNS) and 6 (2.9%) were non- Staphylococcal (rods) isolates. The antibiotic susceptibilities of the Staphylococcus aureus isolates were in the following order: ofloxacin (99.3%) > ciprofloxacin (98.6%) > gentamicin (91.5%) > cephalexine (56%) > sulphamethoxazole/trimethoprim (46.1%) > tetracycline (43.3%) > cefuroxime (33.3%) > cefoxitin (2.1%) > amoxicillin (1.4%) > ampicillin (1.4%). Resistance to three or more antimicrobials was presented in 98.6% of the Staphylococcus aureus isolates. Of the 141 Staphylococcus aureus isolates tested for β-lactamase production, 31 (22.5%) were β lactamase producers. However, of the 31 β-lactamase producers, only 13 (41.9%) were multidrug resistant to cefoxitin, Ampicillin and cephalexine. PCR assay was used to detect mecA and blaZ genes in multi-drug resistant Staphylococcus aureus isolates that were β-lactamase producers. The MDRStaphylococcus aureus was also tested by latex agglutination for presence of PBP2a. PCR results indicated none of the isolates showed any amplification to both mecA gene and blaZ gene. All the Beta-lactamase producing MDR- Staphylococcus aureus isolates tested showed negative reaction to latex agglutination test which was also an indication of absence of Pinicillin binding protein (PBP2a). In conclusion, high isolation of Staphylococcus aureus (70.5%) causing bovine mastitis was observed in this study in which both mecA and blaZ genes were absent





Related Project Materials

THE INFLUENCE OF CULTURAL COMPETENCE ON ADMINISTRATIVE PERFORMANCE

THE INFLUENCE OF CULTURAL COMPETENCE ON ADMINISTRATIVE PERFORMANCE

Abstract: This stud...

Read more
EFFECT OF WEBINAR MULTIMEDIA PLATFORM ON STUDENTS' ACADEMIC PERFORMANCE

EXCERPT FROM THE STUDY

E-learning in education is the wholesome integration of modern telecommunications equipment and I...

Read more
THE IMPACT OF TAXATION ON ECONOMIC GROWTH IN NIGERIA

Abstract

This project is titled the effect of taxation on Nigeria Economic growth. The objective of the...

Read more
Examining the Impact of Social Media on Emergency Response Coordination in Karu Local Government Area, Nasarawa State

Chapter One: Introduction

1.1 Background of the Study...

Read more
The Impact of Governance Reforms on Local Administrative Capacity in Nasarawa Local Government, Nasarawa State

Background of the Study

Governance reforms in Nigeria have been a response to the need for more efficient, transparent,...

Read more
TEACHER QUALITY AND WORK ENVIRONMENT AS DETERMINANTS OF SECONDARY SCHOOL STUDENTS ACADEMIC PERFORMANCE

ABSTRACT

This study was carried out to examine teacher quality and work environment as determinants of secondary school...

Read more
An investigation of public health preparedness for disease outbreaks in Jigawa State

Background of the Study

Public health preparedness refers to the capacity of a region or country to respond effectively to health emergen...

Read more
An Examination of Water Scarcity and Its Impact on Rural Health in Bauchi State

Background of the Study
Water scarcity is a pressing challenge that affects millions of people globally, particularly in ru...

Read more
An Evaluation of Maternal Mortality Reduction Policies in Wase Local Government, Plateau State

Background of the Study

Maternal mortality is a critical public health issue in Nigeria, where the maternal mortality ra...

Read more
Design of an Alumni Management System for Federal College of Education, Zaria

Background of the Study

Alumni management is an essential component of higher education institutions, a...

Read more
Share this page with your friends




whatsapp