0704-883-0675     |      dataprojectng@gmail.com

ANTIMICROBIAL SUSCEPTIBILITY AND MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS FROM MASTITIC COWS

  • Project Research
  • 1-5 Chapters
  • Abstract : Available
  • Table of Content: Available
  • Reference Style: APA
  • Recommended for : Student Researchers
  • NGN 3000

ABSTRACT

This study investigated the antimicrobial susceptibility and molecular characterization of Staphylococcus aureus in mastitic cows in southern part of Kano State of Nigeria. A total of two hundred and thirty four (234) milk and swab samples were collected from six (6) local government areas of Kano State in northern part of Nigeria. Staphylococcus aureus was isolated from samples and identified using standard microbiological procedures. The antimicrobial susceptibility of the isolates was determined using Kirby- Bauer disc diffusion technique as described by the Clinical and Laboratory standards institute (CLSI). Resistant isolates were tested for Beta-lactamase production. A total of 200 (85.5%) have staphylococcal isolates, of which 141 (70.5%) were Staphylococcus aureus (coagulase positive staphylococci, CPS), 59 (29.5%) were coagulase negative staphylococci (CoNS) and 6 (2.9%) were non- Staphylococcal (rods) isolates. The antibiotic susceptibilities of the Staphylococcus aureus isolates were in the following order: ofloxacin (99.3%) > ciprofloxacin (98.6%) > gentamicin (91.5%) > cephalexine (56%) > sulphamethoxazole/trimethoprim (46.1%) > tetracycline (43.3%) > cefuroxime (33.3%) > cefoxitin (2.1%) > amoxicillin (1.4%) > ampicillin (1.4%). Resistance to three or more antimicrobials was presented in 98.6% of the Staphylococcus aureus isolates. Of the 141 Staphylococcus aureus isolates tested for β-lactamase production, 31 (22.5%) were β lactamase producers. However, of the 31 β-lactamase producers, only 13 (41.9%) were multidrug resistant to cefoxitin, Ampicillin and cephalexine. PCR assay was used to detect mecA and blaZ genes in multi-drug resistant Staphylococcus aureus isolates that were β-lactamase producers. The MDRStaphylococcus aureus was also tested by latex agglutination for presence of PBP2a. PCR results indicated none of the isolates showed any amplification to both mecA gene and blaZ gene. All the Beta-lactamase producing MDR- Staphylococcus aureus isolates tested showed negative reaction to latex agglutination test which was also an indication of absence of Pinicillin binding protein (PBP2a). In conclusion, high isolation of Staphylococcus aureus (70.5%) causing bovine mastitis was observed in this study in which both mecA and blaZ genes were absent





Related Project Materials

DETERMINANTS OF DEPOSIT MONEY BANKS’ SHARE PERFORMANCE ON THE NIGERIAN STOCK MARKET

Abstract

Share price is a reflection of a company’s performance and investors’ expectations about the future...

Read more
CRITICAL ANALYSIS OF CORROBORATION UNDER THE NIGERIAN LAW OF EVIDENCE

ABSTRACT

 

There is no law that says the plaintiff or the prosecution must bring a million witnesses or evidence to cour...

Read more
AN EVALUATION OF HEALTH AND SAFETY POLICY IN THE PUBLIC SERVICE

BACKGROUND OF THE STUDY

Occupational Health as defined by the joint committee of the ILO/WHO (1950) is...

Read more
IMPACT OF ADULT EDUCATION ON COMMUNITY DEVELOPMENT

Abstract: The topic of this research is the impact of adult education on community development. This study aimed to explore how adult educatio...

Read more
SCHOOL ENVIRONMENT AND ITS INFLUENCE ON STUDENTS ACADEMIC PERFORMANCE IN PUBLIC SECONDARY SCHOOLS

BACKGROUND OF THE STUDY

Generally, in the whole world, particularly in Nigeria, education has been cons...

Read more
A COMPARATIVE ANALYSIS OF RELATIONSHIP BETWEEN RENTAL RESIDENTIAL AND COMMERCIAL PROPERTIES IN NIGERIA

Background of the study

A home is one of life's most fundamental need. This need can be met for an individual in one...

Read more
THE IMPACT OF SOCIO-ECONOMIC AND CULTURAL FACTORS ON THE USE OF FAMILY PLANNING SERVICES IN NKANU AREA OF ENUGU STATE

Background To The Study

Family Planning is the deliberate regulation of conception or delivery, or the use of contracept...

Read more
ENDSARS PROTEST AND POLITICAL AWARENESS OF STUDENT (A CASE STUDY OF AMBROSE ALLI UNIVERSITY, EKPOMA)

Background of the Study

The Special Anti-Robbery Squad, often known as SARS, is a special police force...

Read more
DEVELOPMENT OF A SHUNT ACTIVE POWER FILTER FOR HARMONIC REDUCTION USING SYNCHRONOUS REFERENCE FRAME WITH SPACE VECTOR PULSE WIDTH MODULATION

ABSTRACT

This work aimed at designing and implementation of Shunt Active Power Filter (SAPF) for power quality improvement through harmon...

Read more
PROBLEMS OF NEW BANKS IN NIGERIA

Abstract

The purpose of the study was to identify challenges facing New banks in Nigeria. A descriptive...

Read more
Share this page with your friends




whatsapp